Rec.Pr.Thyrosin Phosphate (RPTP) anticorps

N° du produit ABIN1042487

L’anticorps Souris Monoclonal anti- a été validé pour FACS, IF, IP et WB. Il convient pour détecter dans des échantillons de Humain, Porc et Rat. Il y a 1 publication disponible.

Aperçu rapide pour Rec.Pr.Thyrosin Phosphate (RPTP) anticorps (ABIN1042487)

Antigène: Rec.Pr.Thyrosin Phosphate (RPTP)

Reactivité: Humain, Porc, Rat

Hôte: Souris

Clonalité: Monoclonal

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Clone: 3D7

Détail du produit

Specificité: Monoclonal antibodies 3G4 and 3D7 have been directed against the extracellular domain of RPTPµ.

Attributs du produit: Cell adhesion

Purification: Purified

Isotype: IgG2a

Information d'application

Indications d'application: 3D7 is useful for western blot, immunofluorescence, immunoprecipitation and flow cytometry. Optimal antibody dilution should be determined by titration.

Commentaires:

3D7 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with ExG protein, secreted by NIH-3T3 transfected cells and purified with glutahione beads. The cells have been transfected using a fusion construct, termed ExG, encoding the entire ectodomain of the human RPTPµ molecule (aa 1-742), fused COOH terminally to GST.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.09% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Référence

Zondag, Reynolds, Moolenaar: "Receptor protein-tyrosine phosphatase RPTPmu binds to and dephosphorylates the catenin p120(ctn)." dans: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11264-9, (2000) (PubMed).

Détail du antigène

Antigène: Rec.Pr.Thyrosin Phosphate (RPTP)

Sujet: Receptor-like protein tyrosine phosphatases (RPTPs) represent a new family of transmembrane proteins that are thought to transduce external signals by dephosphorylating phosphotyrosine residues on intracellular substrates. RPTPµ is a prototypic receptor-like protein-tyrosine phosphatase (RPTP) that mediates homotypic cell-cell interactions. Intracellularly, RPTPµ consists of a relatively large juxtamembrane region (about 150 amino acids) and two conserved phosphatase domains. As in most RPTPs, the first (membrane-proximal) PTP domain of RPTPµ is catalytically active, whereas its second (C-terminal) domain is inactive. RPTPµ is involved in cell-cell adhesion through homophilic interactions and may also play a key role in signal transduction and growth control.